Journal: PLoS Pathogens

Article Title: Porphyromonas gingivalis and Treponema denticola Exhibit Metabolic Symbioses

doi: 10.1371/journal.ppat.1003955

Figure Lengend Snippet: Cell density of P. gingivalis and T. denticola mono- and co-cultures from three independent continuous cultures in OBGM with the dilution rate of 0.044 h −1 and mean generation time of 15.8 h as determined by measuring A 650 nm . The arrow shows the addition of P. gingivalis to a steady state T. denticola culture.

Article Snippet: The 60-mer oligonucleotide probes representing predicted open reading frames (ORFs) of the P. gingivalis and T. denticola genomes to be used for microarray slide preparation were designed using OligoArray 2.l and using the bioinformatic services of Illumina Inc. (CA, USA).

Techniques:

Journal: PLoS Pathogens

Article Title: Porphyromonas gingivalis and Treponema denticola Exhibit Metabolic Symbioses

doi: 10.1371/journal.ppat.1003955

Figure Lengend Snippet: Co-culture was collected, fixed on a coverslip, dehydrated, covered with colloidal silver, gold-coated and imaged using a Philips XL30 field-emission scanning electron microscope. Electron micrographs showed that P. gingivalis and T. denticola coaggregated. T. denticola is a long helical shaped spirochete with an average length of 5 to 20 µm. P. gingivalis is a coccobacillus with an average diameter of 1 µm. Putative T. denticola outer sheath vesicles and/or P. gingivalis outer membrane vesicles are indicated by arrows along the length of T. denticola .

Article Snippet: The 60-mer oligonucleotide probes representing predicted open reading frames (ORFs) of the P. gingivalis and T. denticola genomes to be used for microarray slide preparation were designed using OligoArray 2.l and using the bioinformatic services of Illumina Inc. (CA, USA).

Techniques: Co-Culture Assay, Microscopy, Membrane

Journal: PLoS Pathogens

Article Title: Porphyromonas gingivalis and Treponema denticola Exhibit Metabolic Symbioses

doi: 10.1371/journal.ppat.1003955

Figure Lengend Snippet: T. denticola and P. gingivalis genes differentially expressed during continuous co-culture relative to mono-culture, grouped by COG category.

Article Snippet: The 60-mer oligonucleotide probes representing predicted open reading frames (ORFs) of the P. gingivalis and T. denticola genomes to be used for microarray slide preparation were designed using OligoArray 2.l and using the bioinformatic services of Illumina Inc. (CA, USA).

Techniques:

Journal: PLoS Pathogens

Article Title: Porphyromonas gingivalis and Treponema denticola Exhibit Metabolic Symbioses

doi: 10.1371/journal.ppat.1003955

Figure Lengend Snippet: Expression of T. denticola genes encoding enzymes involved in glycine or glycine-related metabolism during co-culture with P. gingivalis .

Article Snippet: The 60-mer oligonucleotide probes representing predicted open reading frames (ORFs) of the P. gingivalis and T. denticola genomes to be used for microarray slide preparation were designed using OligoArray 2.l and using the bioinformatic services of Illumina Inc. (CA, USA).

Techniques: Expressing

Journal: PLoS Pathogens

Article Title: Porphyromonas gingivalis and Treponema denticola Exhibit Metabolic Symbioses

doi: 10.1371/journal.ppat.1003955

Figure Lengend Snippet: a) The cell numbers of P. gingivalis in different media as determined by absorbance at 650 nm. b) The concentration of free glycine in different P. gingivalis cultures over time, as determined by GC-MS. Data shown are the average of three biological replicates. P. gingivalis grown in:- OBGM – black diamond; OBGM/PBS – black cross; OBGM/ T. denticola conditioned medium – white square. Uninoculated OBGM/ T. denticola conditioned medium – black square.

Article Snippet: The 60-mer oligonucleotide probes representing predicted open reading frames (ORFs) of the P. gingivalis and T. denticola genomes to be used for microarray slide preparation were designed using OligoArray 2.l and using the bioinformatic services of Illumina Inc. (CA, USA).

Techniques: Concentration Assay, Gas Chromatography-Mass Spectrometry

Journal: PLoS Pathogens

Article Title: Porphyromonas gingivalis and Treponema denticola Exhibit Metabolic Symbioses

doi: 10.1371/journal.ppat.1003955

Figure Lengend Snippet: The difference in the amount of free glycine relative to that at t = 0 h as a function of P. gingivalis cell numbers in a) OBGM/PBS, b) OBGM and c) OBGM/ T. denticola conditioned medium. A regression line was fitted using a linear mixed modelling approach. The slope represents the amount of glycine produced/10 9 P. gingivalis cells.

Article Snippet: The 60-mer oligonucleotide probes representing predicted open reading frames (ORFs) of the P. gingivalis and T. denticola genomes to be used for microarray slide preparation were designed using OligoArray 2.l and using the bioinformatic services of Illumina Inc. (CA, USA).

Techniques: Produced

Journal: PLoS Pathogens

Article Title: Porphyromonas gingivalis and Treponema denticola Exhibit Metabolic Symbioses

doi: 10.1371/journal.ppat.1003955

Figure Lengend Snippet: The E. coli TPP auxotrophic strain JW3957-1 (black shading) and the parent strain JRG902 (white shading) were cultured in M9 growth medium (that lacks thiamine) supplemented with either (a) uninoculated P. gingivalis medium (that lacks thiamine) or (b) cell-free P. gingivalis spent medium. The bacterium was cultured with or without TPP addition (5.88 nM).

Article Snippet: The 60-mer oligonucleotide probes representing predicted open reading frames (ORFs) of the P. gingivalis and T. denticola genomes to be used for microarray slide preparation were designed using OligoArray 2.l and using the bioinformatic services of Illumina Inc. (CA, USA).

Techniques: Cell Culture